Authors
Basil P Hubbard, Ahmed H Badran, John A Zuris, John P Guilinger, Kevin M Davis, Liwei Chen, Shengdar Q Tsai, Jeffry D Sander, J Keith Joung, David R Liu
Publication date
2015/10
Journal
Nature methods
Volume
12
Issue
10
Pages
939-942
Publisher
Nature Publishing Group US
Description
Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator–like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
Scholar articles
BP Hubbard, AH Badran, JA Zuris, JP Guilinger… - Nature methods, 2015