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The large spatial heterogeneity in soil physico-chemical and microbial parameters challenges our ability to predict and model pesticide leaching from agricultural land. Microbial mineralization of pesticides is an important process with respect to pesticide leaching since mineralization is the major process for the complete degradation of pesticides without generation of metabolites. The aim of our study was to determine field-scale variation in the potential for mineralization of the herbicides glyphosate, bromoxyniloctanoate, diflufenican, and bentazone and to investigate whether this variation can be predicted by variations in basic soil parameters. Sixty-five soil samples were sampled from an agricultural, loamy field in Silstrup, Denmark, from a 60 × 165 m rectangular grid. The mineralization potential of the four pesticides was determined using a 96-well microplate ¹⁴C-radiorespirometric method. Initial …