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Pine ectomycorrhizae were collected from bedrock and moraine sites in a mature Pinus sylvestris L. forest in the vicinity of Uppsala, Sweden, on four occasions over the period of a year. Ectomycorrhizae were examined microscopically and five characteristic types (Piloderma, tuberculate, Lactarius-like, Russula-like and an unidentified pink) were described and used for further biochemical assays. Total protein, ergosterol and chitin extractions were performed in sequence for each sample of distinct mycorrhizal morphotype. Differences between sites (bedrock moss vs moraine) from which the morphotypes originated were small, except for an increase in protein in Piloderma and tuberculate mycorrhizae on moraine sites in July and January. However, there were significant variations in protein and chitin concentrations and in the ergosterol-to-chitin ratio, both between seasons and mycorrhizal types. Protein …