Authors
Emilie Chancerel, Camille Lepoittevin, Grégoire Le Provost, Yao-Cheng Lin, Juan Pablo Jaramillo-Correa, Andrew J Eckert, Jill L Wegrzyn, Diana Zelenika, Anne Boland, Jean-Marc Frigerio, Philippe Chaumeil, Pauline Garnier-Géré, Christophe Boury, Delphine Grivet, Santiago C González-Martínez, Pierre Rouzé, Yves Van de Peer, David B Neale, Maria T Cervera, Antoine Kremer, Christophe Plomion
Publication date
2011/12
Journal
BMC genomics
Volume
12
Pages
1-14
Publisher
BioMed Central
Description
Background
Single nucleotide polymorphisms (SNPs) are the most abundant source of genetic variation among individuals of a species. New genotyping technologies allow examining hundreds to thousands of SNPs in a single reaction for a wide range of applications such as genetic diversity analysis, linkage mapping, fine QTL mapping, association studies, marker-assisted or genome-wide selection. In this paper, we evaluated the potential of highly-multiplexed SNP genotyping for genetic mapping in maritime pine (Pinus pinaster Ait.), the main conifer used for commercial plantation in southwestern Europe.
Results
We designed a custom GoldenGate assay for 1,536 SNPs detected through the resequencing of gene fragments (707 in vitro SNPs/Indels) and from Sanger-derived Expressed Sequenced Tags assembled into a unigene set (829 in silico …
Scholar articles
E Chancerel, C Lepoittevin, G Le Provost, YC Lin… - BMC genomics, 2011