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Recent literature demonstrates the contribution of short food supply chains (SFSC) to regional economies and sustainable food systems, and acknowledges their role as drivers for sustainable development. Moreover, different types of SFSC have been supported by urban food policies (UFP) over the few last years and actors from the food chain became part of new institutional settings for urban food policies. However, evidence from the sustainability impact assessment (SIA) of these SFSC in urban contexts is limited. Our paper presents an approach for the development of an SIA framework for different SFSC types. In addition, a practical application of the tool in four metropolitan regions (Berlin, London, Ljubljana and Nairobi) is tested. The conceptual development of the SIA framework is based on an in-depth analysis of existing SIA frameworks and methods for assessing the sustainability of agriculture, food …