Authors
Gisela S Alvarez, María L Foglia, Guillermo J Copello, Martín F Desimone, Luis E Diaz
Publication date
2009/3
Journal
Applied microbiology and biotechnology
Volume
82
Pages
639-646
Publisher
Springer Berlin Heidelberg
Description
Immobilized bacteria are being extensively used for metabolite production, biocatalysts, and biosensor construction. However, long-term viability and metabolic activity of entrapped bacteria is affected by several conditions such as their physiological state, the presence of high-osmolarity environments, porous structure and shrinkage of the matrix. The aim of this work was to evaluate the effect of various parameters on bacteria immobilized in sol–gel-derived silica matrices. With this purpose, we evaluated the stress of immobilization over bacteria cultures obtained from different growing states, the effect of cell density and bacteria capability to proliferate inside matrices. Best results to attain longer preservation times were obtained when we immobilized suspensions with an optimized bacterial number of 1 × 107 cfu/gel in the presence of LB medium using aqueous silica precursors. Furthermore, the impact …
Total citations
Scholar articles
GS Alvarez, ML Foglia, GJ Copello, MF Desimone… - Applied microbiology and biotechnology, 2009