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Grayia is a genus of relatively large (1.2–2.5 m) aquatic Afrotropical snakes. Recent molecular phylogenies recovered Grayia in its own distinct subfamily (Grayiinae), which was supported as the sister group to Colubrinae. Tropical African snakes are generally understudied, so the relationships within Grayia are poorly known. High degrees of intra- and interspecies variation can make identification difficult, and previous studies involving Grayia included misidentified specimens in other genera. The goal of this study is to create a phylogenetic tree that can be used to understand the relationships and taxonomy of Grayia via an integrative taxonomic approach that combines molecular data for 60 specimens and morphological data for 719 specimens. Two nuclear (BDNF, NT3) and four mitochondrial genes (COI, cyt b, 16S, and ND4) were used to construct phylogenetic trees with Maximum Likelihood …