Authors
Yuanyuan Chen, Delong Meng, Huibo Wang, Ruochuan Sun, Dongrui Wang, Shuai Wang, Jiajun Fan, Yingjie Zhao, Jingkun Wang, Song Yang, Cong Huai, Xiao Song, Rong Qin, Tao Xu, Dapeng Yun, Lingna Hu, Jingmin Yang, Xiaotian Zhang, Haoming Chen, Juxiang Chen, Hongyan Chen, Daru Lu
Publication date
2015/3/1
Journal
Neuro-oncology
Volume
17
Issue
3
Pages
407-418
Publisher
Oxford University Press
Description
Background. Malignant glioma is a common and lethal primary brain tumor in adults. Here we identified a novel oncoprotein, vesicle-associated membrane protein 8 (VAMP8), and investigated its roles in tumorigenisis and chemoresistance in glioma.
Methods. The expression of gene and protein were determined by quantitative PCR and Western blot, respectively. Histological analysis of 282 glioma samples and 12 normal controls was performed by Pearson’s chi-squared test. Survival analysis was performed using the log-rank test and Cox proportional hazards regression. Cell proliferation and cytotoxicity assay were conducted using Cell Counting Kit-8. Autophagy was detected by confocal microscopy and Western blot.
Results. VAMP8 was significantly overexpressed in human glioma specimens and could become a potential novel prognostic and treatment-predictive marker for glioma patients. Overexpression …
Scholar articles
Y Chen, D Meng, H Wang, R Sun, D Wang, S Wang… - Neuro-oncology, 2015