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Response time (RT) is a critical human factor that influences traffic flow characteristics and traffic safety, and is governed by drivers’ decision-making behavior. Unlike the traditional environment (TE), the connected environment (CE) provides information assistance to drivers. This in-vehicle informed environment can influence drivers’ decision-making and thereby their RTs. Therefore, to ascertain the impact of CE on RT, this study develops RT estimation methodologies for TE (RTEM-TE) and CE (RTEM-CE), using vehicle trajectory data. Because of the intra-lingual inconsistency among traffic engineers, modelers, and psychologists in the usage of the term RT, this study also provides a ubiquitous definition of RT that can be used in a wide range of applications. Both RTEM-TE and RTEM-CE are built on the fundamental stimulus–response relationship, and they utilize the wavelet-based energy distribution of time …