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RNA intcractionsas thc numbcr of availablc protein-RNA coml) lcx thrce-dirnensionalstructuTcs has increased. W6 carry out computational analyses of X6 iepresentative pietein-RNA eomplexes retrieved 1'remPiuteinDaLaBank, InLerfuee rcslduc prepcnsity, which glycsa rncasurc ferthe rclatiyc inipertanccof diffcrcnt amine acid residues inthe RNA inrerfaee. iscaleulated foreach antino acid residue t) pe (residue singlet). In addition to the residue singlet propensity, we intrnducea new residue-based propensiLynamed doubletprepensity, which giyesa measure uf residut pairingprcfercnccsin RNA intcrfaceof a protein. Predictionof RNA interface vv'ith two types of prepensitiesplusa pesitiom-specific mult. iple sequence profileTeaches specificity oF about SO%. The predietionenables us to pninteut candidate RNA interfi] ceresidues. purt of which are consistent with previous experimental studies and may contribute to elucidation of atonric niechanisms oi RNA-bindingpreteins.