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Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

Authors

Michael K Winson, Simon Swift, Leigh Fish, John P Throup, Frieda Jørgensen, Siri Ram Chhabra, Barrie W Bycroft, Paul Williams, Gordon SAB Stewart

Publication date

1998/6/1

Journal

FEMS microbiology letters

Volume

163

Issue

Pages

185-192

Publisher

Blackwell Publishing Ltd

Description

Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to P_luxI, P_lasI and P_rhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.

Total citations

Cited by 736

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Scholar articles

Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing

MK Winson, S Swift, L Fish, JP Throup, F Jørgensen… - FEMS microbiology letters, 1998