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Understanding the interspecific interactions (spatial and temporal) between predators and their prey species is important to understanding the prey preferences for conservation and management decisions. However, due to large predators’ wide-ranging, nocturnal, and cryptic behaviour, it is often difficult to assess their interactions with prey species. Therefore, we determined the spatial and temporal interactions of leopard (Panthera pardus) with potential prey species in Kalesar National Park (KNP) using camera traps from January 2020 to April 2020. KNP is situated in the foothills of the Shiwalik mountain range of Himalaya, North India. We used encounter rates and activity patterns to understand the spatial and temporal overlap between leopards and prey species. We used composite scores to predict the potential prey preferences using the photo-capture data. A total sampling effort of 1150 trap nights …