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Primary biological aerosol particles are ubiquitous in the global atmosphere and can affect cloud formation, deteriorate air quality, and cause human infections. Mt. Tai (1,534 m a.s.l.) is an elevated site in the North China Plain where atmospheric aerosols reflect both regional advection and long‐range transport. In this study, we deployed a Wideband Integrated Bioaerosol Sensor (WIBS‐4A) and collected total suspended particles and eight‐stage size‐segregated aerosol samples at the summit of Mt. Tai in spring from 21 March to 8 April 2017 to quantify the abundance, size distributions, and diurnal variations of fluorescent bioaerosols and to investigate the effect of different fluorescence thresholds of WIBS for ambient bioaerosol recognition. During the whole sampling period, the number concentration of fluorescent particles (>0.8 μm) was 647 ± 533 L⁻¹, accounting for 26.9% ± 10.0% by number of the total …