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The integration of sensitive catalysts in redox matrices opens up the possibility for their protection from deactivating molecules such as O₂. [FeFe]‐hydrogenases are enzymes catalyzing H₂ oxidation/production which are irreversibly deactivated by O₂. Therefore, their use under aerobic conditions has never been achieved. Integration of such hydrogenases in viologen‐modified hydrogel films allows the enzyme to maintain catalytic current for H₂ oxidation in the presence of O₂, demonstrating a protection mechanism independent of reactivation processes. Within the hydrogel, electrons from the hydrogenase‐catalyzed H₂ oxidation are shuttled to the hydrogel–solution interface for O₂ reduction. Hence, the harmful O₂ molecules do not reach the hydrogenase. We illustrate the potential applications of this protection concept with a biofuel cell under H₂/O₂ mixed feed.