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MATERIALS AND METHODS

Materials. Ascorbate, cysteine, Ferrozine [4, 4'-[3-(2-pyridinyl)-l, 2, 4-triazine-5, 6-diyl] bisbenzenesulfonic acid], horse fer-ritin serum antibodies, soybean phosphatidylcholine, and 2, 2'-dipyridyl were purchased from Sigma Chemical Co. Ferritin (horse spleen) was obtained from Boehringer Mannheim Biochemicals. All other chemicals were reagent grade or purer. Methods. Ferritin was separated from loosely bound and unbound iron by dialysis against Chelax resin for 12 h. The iron concentration of dialyzed ferritin was determined by using dipyridyl as described by Drysdale and Munro (1965). The con-centration of iron bound to ferritin in beef psoas major muscle was determined by antibody precipitation of ferritin according to the method ofLinder and Munro (1972). The psoas major muscle used in these experiments was removed from the animals within 2 h of slaughter, chopped in a …