Authors
Christian Heiss, Nicolas Amabile, Andrew C Lee, Wendy May Real, Suzaynn F Schick, David Lao, Maelene L Wong, Sarah Jahn, Franca S Angeli, Petros Minasi, Matthew L Springer, S Katharine Hammond, Stanton A Glantz, William Grossman, John R Balmes, Yerem Yeghiazarians
Publication date
2008/5/6
Journal
Journal of the American College of Cardiology
Volume
51
Issue
18
Pages
1760-1771
Publisher
American College of Cardiology Foundation
Description
Objectives
This study sought to analyze the effects of acute secondhand smoke (SHS) exposure on the number and function of endothelial progenitor cells (EPCs) over 24 h.
Background
Secondhand smoke increases the risk of vascular disease and is a major public health concern, but the mechanism(s) of action are not fully understood.
Methods
Healthy nonsmokers (age SEM 30.3 ± 1.3 years, n = 10) were exposed to 30 min of SHS yielding cotinine levels commonly observed in passive smokers and to smokefree air on 2 separate days. Measurements were taken before exposure (baseline), immediately after (0 h), and at 1 h, 2.5 h, and 24 h after. The EPCs (CD133+/KDR+, CD34+/KDR+) and endothelial microparticles (EMPs: CD31+/CD41−, CD144+, CD62e+) were determined in blood using flow cytometry. The EPC chemotaxis toward vascular endothelial growth factor was measured. Endothelial function was …
Total citations
Scholar articles
C Heiss, N Amabile, AC Lee, WM Real, SF Schick… - Journal of the American College of Cardiology, 2008