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The genus Cytinus, recognised as one of the most enigmatic in the plant kingdom, has garnered attention for its bioactive potential, particularly its skin anti-ageing properties. Despite this recognition, much remains to be accomplished regarding deciphering and isolating its most active compounds.

This study aimed to identify the compounds responsible for C. hypocistis skin anti-ageing potential.

Using multivariate analysis, a biochemometric approach was applied to identify the discriminant metabolites by integrating extracts' chemical profile (Liquid Chromatography–High-Resolution Mass Spectrometry, LCsingle bondHRMS) and bioactive properties. The identified bioactive metabolite was structurally elucidated by 1D and 2D Nuclear Magnetic Resonance (NMR).

Among the studied bioactivities, the anti-elastase results exhibited a significant variation among the samples from …