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Nowadays, it is recognized the need for improved safety and efficacy protocols to evaluate the human stratum corneum (SC) and its interaction with topical and cosmetic formulations by minimally or non‐invasive methodologies. The aim of our research work was to streamline the HPLC‐TBARS‐EVSC (high‐performance liquid chromatography–thiobarbituric acid reactive substances–ex vivo stratum corneum) methodology, by exploring the results of a group of 18 subjects.

The study included nine women and nine men aged between 19 and 57 years old with phototypes from II to V. Sites in the forearm of each volunteer were randomly delimited, and the SC was collected by tape stripping. HPLC was used to quantify the MDA‐TBA₂ (malondialdehyde–thiobarbituric acid) adduct from the tape‐stripped SC, irradiated and not by an ultraviolet (UV) simulator chamber.

Observing the …