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Exposure to carefully selected information on social networking sites (SNSs) showing a flawless self and an ideal life has been found to harm young individuals’ self-esteem and well-being. However, SNS use is no longer limited to young people. Fueled by the sharp increase of smartphone ownerships, which allow users to access information about others anytime and anywhere, nowadays, a wide range of age groups uses SNSs. Accordingly, this study investigated the relationships between mobile SNS use, upward social comparison, self-esteem, and well-being for the first time. We employed a two-wave panel survey (N_T2 = 461) using a quota sample of adults. Results revealed that Facebook use predicted upward social comparison, which harmed individuals’ self-esteem and well-being over time, while Instagram use directly influenced well-being negatively over time. Additionally, we found support for …