Authors
Ali Amiryousefi, Jaakko Hyvönen, Péter Poczai
Publication date
2018/6
Journal
Applications in plant sciences
Volume
6
Issue
6
Pages
e01159
Description
Premise of the Study
To accurately design plant genetic studies, the information content of utilized markers and primers must be calculated. Plant genotyping studies should take into account the efficiency of each marker system by calculating different parameters to find the optimal combination of primers. This can be problematic because there are currently no easily accessible applications that can be used to calculate multiple indices together.
Methods and Results
The program Online Marker Efficiency Calculator (iMEC) was developed using R for the simple computation of seven polymorphism indices (heterozygosity index, polymorphism information content, discriminating power, effective multiplex ratio, marker index, arithmetic mean heterozygosity, and resolving power). These indices are based on dominant and codominant DNA fingerprinting markers, thus allowing comparison and selection of optimal …
Total citations
2018201920202021202220232024171932414821
Scholar articles
A Amiryousefi, J Hyvönen, P Poczai - Applications in plant sciences, 2018