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Geochemical analyses were used to classify 39 Zechstein (Late Permian, Lopingian) Main Dolomite (Ca2) crude oil samples from fields in the eastern and southern sector of the Southern Permian Basin (SPB) of Europe and to provide new insights into the origin of the oil. Geochemical data indicate that Ca2 oils were generated in the early-to-late oil window and are mostly non-waxy oils. Various biomarker and stable carbon isotopic ratios were used to identify source and depositional settings for source rocks of Ca2 oils arranged within 10 distinct oil groups. Specifically, the geochemical analyses and oil-oil correlations revealed a set of characteristic biomarkers including an even-over-odd predominance (EOP) for the C_20-30 n-alkanes, C₄₀ carotenoid occurrence (isorenieratane, chlorobactane, β-isorenieratane), bisnorhopane/hopane (BNH/H) ratios >0.1, high abundances of C₃₅ homohopanes and elevated …