Authors
Y Wang, XF Chen, XL Xie, M Xiao, Y Yang, G Zhang, JJ Zhang, SM Duan, Q Zhang, P Zhang, C Tsui, YC. Xu
Publication date
2019/6
Journal
Journal of Microbiology, Immunology and Infection
Volume
52
Issue
3
Pages
456-464.doi.org/10.1016/j.jmii.2018.12.0
Description
Background and purpose
Bacteroides fragilis group isolates are most frequently isolated anaerobic pathogens. This study aimed to evaluate the accuracy of VITEK MS, Clin-ToF-II MS, Autof MS 1000 and VITEK 2 ANC card on the identification of clinical B. fragilis group isolates, as well as to determine their antimicrobial susceptibilities.
Methods
A total of 138 isolates of B. fragilis group isolates were identified with the three MALDI-TOF MS systems and VITEK 2 ANC cards. 16S rRNA gene sequencing was used as the reference identification method for comparison. Antimicrobial susceptibilities were determined by agar dilution method to 19 antimicrobial agents recommended by Clinical and Laboratory Standards Institute (CLSI).
Results
Hundred thirty three isolates of Bacteroides spp. and 5 isolates of Parabacteroides spp. were identified by 16S rRNA sequencing. The rates of accurate identification at species level of …
Scholar articles
N Sasaki, S Katagiri, R Komazaki, K Watanabe… - Frontiers in Microbiology, 2018
Y Wang, XF Chen, XL Xie, M Xiao, Y Yang, G Zhang… - Journal of Microbiology, Immunology and Infection, 2019