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Longitudinal and temporal distribution patterns of freshwater ascomycetes and dematiaceous hyphomycetes on submerged wood were investigated at 3 sites along the Lam Tsuen River in Hong Kong. Two-hundred-and-six species were recorded. Ascomycete genera were dominated by Annulatascaceae, Halosphaeriaceae, Lasiosphaeriaceae, Lophiostomataceae, and Magnaporthaceae; the most common taxa were Aniptodera chesapeakensis, Aquaticola ellipsoidea, Helicosporium griseum, Massarina purpurascens, M. thalassioidea, Ophioceras commune, and Sporoschisma uniseptatum. There were no significant differences (ANOVA, p > 0.05) in total number of species among the sites. However, the number of species of Halosphaeriaceae (p < 0.01) and Lasiosphaeriaceae (p < 0.05) was significantly higher at Site 3, and Annulatascaceae was dominant at Sites 1 and 2 (p < 0.05). Downstream changes in …