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Glutathione S-transferases (GSTs) play an important role in the protection of cells against xenobiotics and lipid hydroperoxides generated by oxidative stress. In human, the GSTP1-1 expression is commonly increased in many tumors and involved in the development of antineoplastic drug resistance. Reactive oxygen species are released at inflammation sites and oxidative stress conditions enhance the expression of genes encoding antioxidant enzymes such as GSTs. Here we investigated the regulation of the GSTP1-1 gene expression in the K562 cell line by nuclear factor κB (NF-κB) and the pro-inflammatory cytokine tumor necrosis factor alpha (TNFα). By studying GSTP1-1 mRNA expression and NF-κB/GSTP1-1 promoter interactions, we showed the implication of NF-κB in the GSTP1-1 gene expression and we described a new specific TNFα-inducible NF-κB binding site upstream of the minimal promoter …