Authors
Gabriel O James, Charles H Hocart, Warwick Hillier, Hancai Chen, Farzaneh Kordbacheh, G Dean Price, Michael A Djordjevic
Publication date
2011/2/1
Journal
Bioresource technology
Volume
102
Issue
3
Pages
3343-3351
Publisher
Elsevier
Description
The Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants, BAF-J5 and I7, produce significantly elevated levels of 16:0, 18:1Δ9, 18:2Δ9,12 and 18:3Δ9,12,15 fatty acids. Long-chain saturated, mono- and polyunsaturated fatty acids were found under nitrogen starvation. Oleosin-like and caleosin-like genes were identified in the C. reinhardtii genome. However, proteomic analysis of isolated lipid bodies only identified a key lipid droplet associated protein. This study shows it is possible …
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Scholar articles
GO James, CH Hocart, W Hillier, H Chen… - Bioresource technology, 2011