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Gelatin-based zinc oxide nanoparticle (ZnO NPs) nanocomposite films were prepared using various types of ZnO NPs, which were synthesized using two different zinc salts (zinc nitrate and zinc acetate) with or without capping agent (carboxymethyl cellulose). The ZnO NPs were in various shapes and sizes ranged from 200 to 400 nm depending on the preparation method. The gelatin/ZnO NPs nanocomposite films were characterized by UV–visible spectroscopy, FE-SEM, FT-IR, thermogravimetric analysis, and XRD. The influence of different types of ZnO NPs on the morphology, crystallization behavior, thermal stability, mechanical, water vapor barrier, water contact angle, and antibacterial properties of the gelatin-based nanocomposite films were investigated. Gelatin/ZnO NPs nanocomposite films exhibited three absorption peaks around 220–230, 270–280, and 360 nm. Apparent surface color and transmittance …