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Yield and physicochemical characteristics of protein isolates recovered from rohu processing waste by acid- and alkali-aided protein solubilization methods were studied. pH 2.0 from acidic side and pH 13.0 from alkaline side resulted in maximum protein solubility, whereas minimum solubility was observed at pH 5.5. Except for pH 13.0, theoretical protein recovery was more than the actual recovery after centrifugation. Maximum protein recovery after the first centrifugation cycle was observed for pH 2.0 and pH 13.0, and the maximum protein recovery during the second centrifugation cycle was observed at precipitation pH 5.5 in both acidic and alkaline methods. Acidic and alkaline methods resulted in a total process recovery of 31.81% ± 0.001% and 31.11% ± 0.01%, respectively. Isolates prepared by acidic method had more whiteness and high foaming capacity, whereas isolates prepared using alkaline methods …