Authors
Guy R Simpson, Thomas F Schulz, Denise Whitby, Pamela M Cook, Chris Boshoff, Lucille Rainbow, Mark R Howard, Shou-Jiang Gao, Roy A Bohenzky, Peter Simmonds, Christine Lee, Annemiek de Ruiter, Angelos Hatzakis, Richard S Tedder, Ian VD Weller, Robin A Weiss, Patrick S Moore
Publication date
1996/10/26
Journal
The Lancet
Volume
348
Issue
9035
Pages
1133-1138
Publisher
Elsevier
Description
Background
Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8, may be the infectious cause of KS. Its prevalence in the general population, on the basis of detection of the virus genome, is controversial. To investigate the seroprevalence, we measured antibodies to a recombinant capsid-related (lytic cycle) KSHV antigen and a latent antigen complex.
Methods
We selected potentially immunoreactive capsidrelated proteins of KSHV by expressing them as recombinant proteins and testing them in western blot assays. We used a truncated recombinant protein encoded by KSHV open reading frame 65 (orf 65) to develop a diagnostic enzyme-linked immunosorbent assay (ELISA) and tested sera from HIV-infected individuals with KS, HIV-uninfected patients with "classic" KS, other HIV risk groups, and blood donors. We also compared the antibody response to this capsid-related …
Total citations
Scholar articles
GR Simpson, TF Schulz, D Whitby, PM Cook, C Boshoff… - The Lancet, 1996