Authors
Michael GK Jones, William H Outlaw Jr, Oliver H Lowry
Publication date
1977/9/1
Journal
Plant physiology
Volume
60
Issue
3
Pages
379-383
Publisher
American Society of Plant Biologists
Description
Procedures are described for measuring sucrose in plant extracts or freeze-dried tissue in the range between 10−7 and 10−14 moles. The method is based on the destruction of pre-existing glucose and fructose, followed by the hydrolysis of sucrose and reduction of NADP+ by a series of coupled enzymic reactions. Depending on the sensitivity required, the NADPH is determined directly with a spectrophotometer or a fluorometer, or is amplified as much as 30,000 times before fluorometric assay. The procedures suggested for the macro level are simpler than current methods, and those suggested for microanalysis are several orders of magnitude more sensitive.
With this technique, single palisade parenchyma cells and single spongy parenchyma cells of Vicia faba leaflets were each found to contain about 2.2 pmoles of sucrose.
Total citations
Scholar articles
MGK Jones, WH Outlaw Jr, OH Lowry - Plant physiology, 1977
MGK Jones, WH Outlaw Jr, OM Lowry - Plant Physiol, 1977