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Temperature dependence of reductive trichloroethene (TCE) dechlorination was investigated in an enrichment culture (KB-1™), using lactate or propionate as electron donors at a temperature interval from 4 to 60°C. Dechlorination was complete to ethene at temperatures between 10 and 30°C (lactate-amended) and between 15 and 30°C (propionate-amended). Dechlorination stalled at cis-1,2-dichloroethene (cDCE) at 4°C (lactate-amended), at and below 10°C (propionate-amended), and at 40°C with both electron donors. No dechlorination of TCE was observed at 50 and 60°C. Concentrations of Dehalococcoides had increased or remained constant after 15 days of incubation at temperatures involving complete dechlorination to ethene. Temperature dependence of dechlorination rates was compared using zero order degradation kinetics and a Monod growth-rate model for multiple electron acceptor usage …