Authors
Leon FA van Dullemen, Eelke M Bos, Theo A Schuurs, Harm H Kampinga, Rutger J Ploeg, Harry van Goor, Henri GD Leuvenink
Publication date
2013/12
Journal
Journal of translational medicine
Volume
11
Pages
1-10
Publisher
BioMed Central
Description
Background
Kidneys derived from brain dead donors have lower graft survival and higher graft-function loss compared to their living donor counterpart. Heat Shock Proteins (HSP) are a large family of stress proteins involved in maintaining cell homeostasis. We studied the role of stress-inducible genes Heme Oxygenase-1 (HO-1), HSP27, HSP40, and HSP70 in the kidney following a 4 hour period of brain death.
Methods
Brain death was induced in rats (n=6) by inflating a balloon catheter in the epidural space. Kidneys were analysed for HSPs using RT-PCR, Western blotting, and immunohistochemistry.
Results
RT-PCR data showed a significant increase in gene expression for HO-1 and HSP70 in kidneys of brain dead rats. Western blotting revealed a massive increase in HO-1 protein in brain dead rat kidneys …
Scholar articles
LFA van Dullemen, EM Bos, TA Schuurs… - Journal of translational medicine, 2013