Authors
Joerg M Buescher, Maciek R Antoniewicz, Laszlo G Boros, Shawn C Burgess, Henri Brunengraber, Clary B Clish, Ralph J DeBerardinis, Olivier Feron, Christian Frezza, Bart Ghesquiere, Eyal Gottlieb, Karsten Hiller, Russell G Jones, Jurre J Kamphorst, Richard G Kibbey, Alec C Kimmelman, Jason W Locasale, Sophia Y Lunt, Oliver DK Maddocks, Craig Malloy, Christian M Metallo, Emmanuelle J Meuillet, Joshua Munger, Katharina Nöh, Joshua D Rabinowitz, Markus Ralser, Uwe Sauer, Gregory Stephanopoulos, Julie St-Pierre, Daniel A Tennant, Christoph Wittmann, Matthew G Vander Heiden, Alexei Vazquez, Karen Vousden, Jamey D Young, Nicola Zamboni, Sarah-Maria Fendt
Publication date
2015/8/1
Source
Current opinion in biotechnology
Volume
34
Pages
189-201
Publisher
Elsevier Current Trends
Description
Highlights
- 13 C tracer analysis can be used to probe intracellular metabolism in cells.
- Steady state labeling patterns can quantify nutrient contributions.
- Steady state labeling patterns can inform about pathway activities.
- Dynamic labeling patterns combined with metabolite levels can quantify some fluxes.
- Analysis of multiple metabolites and various tracers enhances information output.
Measuring intracellular metabolism has increasingly led to important insights in biomedical research. 13 C tracer analysis, although less information-rich than quantitative 13 C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting 13 C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state …
Scholar articles
JM Buescher, MR Antoniewicz, LG Boros, SC Burgess… - Current opinion in biotechnology, 2015