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This study evaluates a three‐week online self‐training intervention teaching mindfulness as a cognitive–emotional segmentation strategy. Daily effects on psychological detachment, affective well‐being, psychological and strain‐based work–family conflict, and satisfaction with work–life balance were assessed, with a particular focus on whether segmentation preferences moderate training responsiveness. A randomized wait‐list control group design was used for administering daily questionnaires to 190 participants. Psychological detachment, affective well‐being, and work–life interface measures were assessed daily. As expected, growth curve analyses revealed positive effects on psychological detachment, psychological work–family conflict, and work–life balance satisfaction. No effects were found for strain‐based work–family conflict. Additionally, segmentation preferences moderated the intervention effect on …