Authors
Arthur Georges, Qiye Li, Jinmin Lian, Denis O’Meally, Janine Deakin, Zongji Wang, Pei Zhang, Matthew Fujita, Hardip R Patel, Clare E Holleley, Yang Zhou, Xiuwen Zhang, Kazumi Matsubara, Paul Waters, Jennifer A Marshall Graves, Stephen D Sarre, Guojie Zhang
Publication date
2015/12/1
Journal
Gigascience
Volume
4
Issue
1
Pages
s13742-015-0085-2
Publisher
Oxford University Press
Description
Background
The lizards of the family Agamidae are one of the most prominent elements of the Australian reptile fauna. Here, we present a genomic resource built on the basis of a wild-caught male ZZ central bearded dragon Pogona vitticeps.
Findings
The genomic sequence for P. vitticeps, generated on the Illumina HiSeq 2000 platform, comprised 317 Gbp (179X raw read depth) from 13 insert libraries ranging from 250 bp to 40 kbp. After filtering for low-quality and duplicated reads, 146 Gbp of data (83X) was available for assembly. Exceptionally high levels of heterozygosity (0.85 % of single nucleotide polymorphisms plus sequence insertions or deletions) complicated assembly; nevertheless, 96.4 % of reads mapped back to the assembled scaffolds, indicating that the assembly included most of the sequenced genome. Length of the assembly was 1.8 Gbp in 545,310 …
Scholar articles
A Georges, Q Li, J Lian, D O'Meally, J Deakin, Z Wang… - Gigascience, 2015