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In the present study, we investigated the effect of dietary supplementation with microalgae rich in DHA (C22:6n3) on the fatty acid composition of different fat deposits and muscles in light lambs. Two dietary treatments were studied: control (CT) and microalgae (MT), containing 2% of DHA- rich microalgae. Dietary incorporation of microalgae modified fatty acid composition in all anatomical locations studied (intramuscular, subcutaneous and kidney knob and channel fat); however, intramuscular fat was the most affected deposit. Intramuscular fat of MT lambs had higher levels of DHA (3.35%) and total n3 fatty acids (5.71%), than that of CT lambs (0.25 and 1.23% respectively). Dietary supplementation with microalgae produced a greater proportion of DHA and total n3 in M. Infraspinatus (IM) (5.12 and 8.13% respectively) compared with M. Longissimus (LM) (3.35 and 5.71% respectively) and M. Psoas major (PM) (3 …