Authors
Daryan R Namba, Garret Ma, Idris Samad, Dacheng Ding, Vinciya Pandian, Jonathan D Powell, Maureen R Horton, Alexander T Hillel
Publication date
2015/5
Journal
Otolaryngology--Head and Neck Surgery
Volume
152
Issue
5
Pages
881-888
Publisher
SAGE Publications
Description
Objective
To determine if rapamycin inhibits the growth, function, and metabolism of human laryngotracheal stenosis (LTS)–derived fibroblasts.
Study Design
Controlled in vitro study.
Setting
Tertiary care hospital in a research university.
Subjects and Methods
Fibroblasts isolated from biopsies of 5 patients with laryngotracheal stenosis were cultured. Cell proliferation, histology, gene expression, and cellular metabolism of LTS-derived fibroblasts were assessed in 4 conditions: (1) fibroblast growth medium, (2) fibroblast growth medium with dimethylsulfoxide (DMSO), (3) fibroblast growth medium with 10−10 M (low-dose) rapamycin dissolved in DMSO, and (4) fibroblast growth medium with 10−9 M (high-dose) rapamycin dissolved in DMSO.
Results
The LTS fibroblast count and DNA concentration were reduced after treatment with high-dose rapamycin compared to DMSO (P = .0007) and normal (P = .0007) controls …
Total citations
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Scholar articles
DR Namba, G Ma, I Samad, D Ding, V Pandian… - Otolaryngology--Head and Neck Surgery, 2015