Authors
Alexander T Hillel, Daryan Namba, Dacheng Ding, Vinciya Pandian, Jennifer H Elisseeff, Maureen R Horton
Publication date
2014/10/1
Journal
JAMA Otolaryngology–Head & Neck Surgery
Volume
140
Issue
10
Pages
961-966
Publisher
American Medical Association
Description
Importance
Laryngotracheal stenosis (LTS) lacks an ideal animal model to study its unique wound-healing pathophysiology and the effect of interventions.
Objective
To present an in vivo, in situ mouse model of LTS that can be used to investigate its pathophysiology, mechanisms, and interventions for treatment.
Design, Setting, and Subjects
Prospective controlled animal study performed at an academic animal research facility on 87 C57BL/6 mice.
Interventions
Experimental mice (n = 40) underwent bleomycin-coated wire-brush injury to the larynx and trachea, while mechanical injury controls (n = 32) underwent phosphate-buffered saline–coated wire-brush injury. Normal controls (n = 9) underwent no intervention, and mock surgery controls (n = 6) underwent anterior transcervical tracheal exposure only. Laryngotracheal complexes were harvested at days 7, 14, and 21 after injury. At the respective time …
Scholar articles
AT Hillel, D Namba, D Ding, V Pandian, JH Elisseeff… - JAMA Otolaryngology–Head & Neck Surgery, 2014