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We report two studies that investigate single‐response situational judgment tests (SJTs) as measures of job knowledge. Study 1 examines relationships between job knowledge measured by a single‐response SJT, personality, and performance for museum tour guides. Study 2 extends Study 1's findings with a sample of volunteers using a single‐response SJT about volunteerism. In both studies, personality was related to knowledge, and knowledge predicted performance. In Study 2, knowledge accounted for incremental variance in performance beyond personality, but personality added no incremental variance beyond knowledge. Results suggest that knowledge of effective behavior and knowledge of ineffective behavior are separate constructs. These studies demonstrate the validity of single‐response SJTs and provide evidence that knowing what to do and what not to do are separate domains.