Authors
Masahiko Kobayashi, Ginette R Squires, Aisha Mousa, Michael Tanzer, David J Zukor, John Antoniou, Ulrich Feige, A Robin Poole
Publication date
2005/1
Journal
Arthritis & Rheumatism: Official Journal of the American College of Rheumatology
Volume
52
Issue
1
Pages
128-135
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Description
Objective
To determine whether interleukin‐1 (IL‐1) or tumor necrosis factor α (TNFα), or both, plays a role in the excessive degradation that is observed in cultured osteoarthritic (OA) articular cartilage.
Methods
Antagonists of IL‐1 and TNFα, namely, IL‐1 receptor antagonist and the PEGylated soluble TNFα receptor I, respectively, were added at different concentrations to explant cultures of nonarthritic (5 obtained at autopsy) and OA (15 obtained at arthroplasty) articular cartilage. The cleavage of type II collagen (CII) by collagenase was measured by an immunoassay in cartilage and culture media. Proteoglycan (mainly aggrecan) content and degradation were measured by a colorimetric assay for glycosaminoglycan (GAG) content in cartilage and culture media. Reverse transcriptase–polymerase chain reaction was used to analyze gene expression of matrix metalloproteases (MMPs) 1, 3, and 13, CII …
Total citations
Scholar articles
M Kobayashi, GR Squires, A Mousa, M Tanzer… - Arthritis & Rheumatism: Official Journal of the American …, 2005