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Ensemble size is critical to the efficiency and performance of the ensemble Kalman filter, but when the ensemble size is small, the Kalman gain generally cannot be well estimated. To reduce the negative effect of spurious correlations, a regularization process applied on either the covariance or the Kalman gain seems to be necessary. In this paper, we evaluate and compare the estimation errors when two regularization methods including the distance-dependent localization and the bootstrap-based screening are applied on the covariance and on the Kalman gain. The investigations were carried out through two examples: 1D linear problem without dynamics but for which the true Kalman gain can be computed and a 2D highly nonlinear reservoir fluid flow problem. The investigation resulted in three primary conclusions. First, if localizations of two covariance matrices are not consistent, the estimate of the …