Authors
Adam Wood, Nevan J Krogan, Jim Dover, Jessica Schneider, Jonathan Heidt, Marry Ann Boateng, Kimberly Dean, Ashkan Golshani, Yi Zhang, Jack F Greenblatt, Mark Johnston, Ali Shilatifard
Publication date
2003/1/1
Journal
Molecular cell
Volume
11
Issue
1
Pages
267-274
Publisher
Elsevier
Description
Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.
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Scholar articles
A Wood, NJ Krogan, J Dover, J Schneider, J Heidt… - Molecular cell, 2003