Authors
Vasco Elbrecht, Ecaterina Edith Vamos, Kristian Meissner, Jukka Aroviita, Florian Leese
Publication date
2017/10
Journal
Methods in Ecology and Evolution
Volume
8
Issue
10
Pages
1265-1275
Description
- DNA metabarcoding holds great promise for the assessment of macroinvertebrates in stream ecosystems. However, few large‐scale studies have compared the performance of DNA metabarcoding with that of routine morphological identification.
- We performed metabarcoding using four primer sets on macroinvertebrate samples from 18 stream sites across Finland. The samples were collected in 2013 and identified based on morphology as part of a Finnish stream monitoring program. Specimens were morphologically classified, following standardised protocols, to the lowest taxonomic level for which identification was feasible in the routine national monitoring.
- DNA metabarcoding identified more than twice the number of taxa than the morphology‐based protocol, and also yielded a higher taxonomic resolution. For each sample, we detected more taxa by metabarcoding than by the morphological method, and …
Scholar articles
V Elbrecht, EE Vamos, K Meissner, J Aroviita, F Leese - Methods in Ecology and Evolution, 2017