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The aim of this study was to examine gender differences in match performance characteristics of elite soccer players. Fifty-four male and fifty-nine female soccer players were tracked during UEFA Champions League matches using a multi-camera system (Amisco, Nice, France). Male players covered more (P < .01) distance than female players in total during a match (Effect Size [ES]: 0.5) and at higher speed thresholds (>15, >18, 18–21, 21–23, 23–25 and >27 km h⁻¹; ES: 0.7–1.4). Decrements in the second versus first half (P < .01) were only evident in female players for the distance covered in total and at selected speed thresholds (12–15, >12 and >15 km h⁻¹; ES: 0.6). Male central midfielders covered more (P < .01) total distance during a match than female central midfielders and at selected speed thresholds (15–23 km h⁻¹; ES: 1.3–2.2). Male full-backs and wide midfielders covered a greater distance (P < .01 …