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We examined habitat use by bats in the boreal forest of central Saskatchewan during summer 1995 by monitoring echolocation calls across forest types and through the canopy of mature forest. We sampled bat activity in mature aspen (Populus tremuloides)-white spruce (Picea glauca) mixedwood, aspen, and jack pine (Pinus banksiana) stands and recorded passes from little brown (Myotis lucifugus), northern long-eared (M. septentrionalis), big brown (Eptesicus fuscus) or silver-haired bats (Lasionycteris noctivagans), and hoary (Lasiurus cinereus) bats. There were significantly more bat passes per night in the aspen-white spruce mixedwood forest than in aspen or jack pine forest. There was no difference in the proportion of feeding buzzes recorded from aspen-white spruce mixedwood and aspen forest. In aspen forest, mean number of hoary bat calls per night was higher above than below the canopy …