Authors
Raffaella Giavazzi, Marco Foppolo, Romina Dossi, Andrea Remuzzi
Publication date
1993/12/1
Journal
The Journal of clinical investigation
Volume
92
Issue
6
Pages
3038-3044
Publisher
American Society for Clinical Investigation
Description
We investigated the interaction of different human tumor types with resting and IL-1-activated human umbilical vein endothelial cells under laminar flow conditions using a parallel plate flow chamber. Three tumor cell lines (the HT-29M colon carcinoma, the OVCAR-3 ovarian carcinoma, and the T-47D breast carcinoma) showed limited adhesion to unstimulated endothelial cells at any of the shear stress levels tested, while rolling and massive adhesion of tumor cells were observed on IL-1-activated endothelial cells. Three other tumor cell lines (the A375M and A2058 melanomas and the MG-63 osteosarcoma) did not adhere on resting endothelial cells at high shear stress (> 1.5 dyn/cm2) and started to adhere with decreasing shear stress; the number of adherent cells increased steeply on IL-1-activated endothelial cells, but no cell rolling was observed even at the highest shear stress. These mechanisms of tumor …
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Scholar articles
R Giavazzi, M Foppolo, R Dossi, A Remuzzi - The Journal of clinical investigation, 1993