Authors
John W Pollard, Claire Plante, W Allan King, Peter J Hansen, Keith J Betteridge, Susan S Suarez
Publication date
1991/1/1
Journal
Biology of reproduction
Volume
44
Issue
1
Pages
102-107
Publisher
Oxford University Press
Description
The ability of the bovine oviduct to maintain the motility and fertilizing capacity of bovine sperm was investigated by incubating frozen-thawed sperm with endosalpingeal epithelial cells cultured on either tissue culture plastic (nonpolarizing) or Matrigel-coated Millicell (polarizing) substrata. Sperm were also incubated in medium alone or with cultured bovine tracheal epithelial cells. Motility was determined at 6-h intervals over a 48-h period. The fertilizing capacity of sperm was evaluated after 0, 24, and 30 h of incubation by adding oocytes to the culture and determining the incidences of fertilization and polyspermy. Motility was maintained for 48 h in sperm that bound to endosalpingeal epithelial cells, but to a greater extent with polarized cells (38.4% motile) than with nonpolarized cells (0.8%). Fertilizing capacity was maintained for 30 h in sperm incubated with endosalpingeal epithelial cells on Matrigel/Millicell …
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JW Pollard, C Plante, W Allan King, PJ Hansen… - Biology of reproduction, 1991