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Polycyclic aromatic hydrocarbons (PAHs) are persistent and commonly detected in marine ecosystem. They coexist with a various contaminants including heavy metals in real environment, but most studies have been still focused on single effects of these contaminants to aquatic organisms. In this study, the single and combined effects of benzy [a] pyrene (B [a] P) and HgCl 2 were investigated in the brackish water flea, Diaphanosoma celebensis. For combined effect study, three mixtures A (3: 7), B (5: 5), and C (7: 3) of benzy [a] pyrene (B [a] P) and HgCl 2 were determined by 48 h-LC 50 values of single exposure. Combined effects of both chemicals were estimated by concentration addition (CA) model and independent action (IA) model. As results, 48 h-LC 50 values of benzy [a] pyrene (B [a] P) and HgCl 2 was 25.75㎍ l-1 and 3.6㎍ l-1, respectively. TU values of mixture A, B, and C were 1.06, 0.83, and 0.96, respectively, indicating additive effects of mixtures using CA model. This study will be helpful as basic data for understanding the combined effects of contaminants in marine environment.