Authors
Andrej Shevchenko, Henrik Tomas, Jan Havli, Jesper V Olsen, Matthias Mann
Publication date
2006/12
Journal
Nature protocols
Volume
1
Issue
6
Pages
2856-2860
Publisher
Nature Publishing Group UK
Description
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.
Total citations
Scholar articles
A Shevchenko, H Tomas, J Havli, JV Olsen, M Mann - Nature protocols, 2006