Authors
Guillaume Rigaut, Anna Shevchenko, Berthold Rutz, Matthias Wilm, Matthias Mann, Bertrand Séraphin
Publication date
1999/10
Journal
Nature biotechnology
Volume
17
Issue
10
Pages
1030-1032
Publisher
Nature Publishing Group
Description
We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
Total citations
Scholar articles
G Rigaut, A Shevchenko, B Rutz, M Wilm, M Mann… - Nature biotechnology, 1999