Authors
Andrew J Simkin, Karel Miettinen, Patricia Claudel, Vincent Burlat, Grégory Guirimand, Vincent Courdavault, Nicolas Papon, Sophie Meyer, Stéphanie Godet, Benoit St-Pierre, Nathalie Giglioli-Guivarc’h, Marc JC Fischer, Johan Memelink, Marc Clastre
Publication date
2013/1
Journal
Phytochemistry
Volume
85
Pages
36-43
Publisher
Pergamon
Description
Madagascar periwinkle (Catharanthus roseus [L.] G. Don, Apocynaceae) produces monoterpene indole alkaloids (MIAs), secondary metabolites of high interest due to their therapeutic value. A key step in the biosynthesis is the generation of geraniol from geranyl diphosphate (GPP) in the monoterpenoid branch of the MIA pathway. Here we report on the cloning and functional characterization of C. roseus geraniol synthase (CrGES). The full-length CrGES was over-expressed in Escherichia coli and the purified recombinant protein catalyzed the conversion of GPP into geraniol with a Km value of 58.5μM for GPP. In vivo CrGES activity was evaluated by heterologous expression in a Saccharomyces cerevisiae strain mutated in the farnesyl diphosphate synthase gene. Analysis of culture extracts by gas chromatography-mass spectrometry confirmed the excretion of geraniol into the growth medium. Transient …
Total citations
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Scholar articles
AJ Simkin, K Miettinen, P Claudel, V Burlat… - Phytochemistry, 2013